FastQCFastQC Report
Fri 10 Feb 2017
SRR3559793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608096
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445622.7711032177183452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210591.3095611207291107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154360.9598929417149226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81500.506810538674308No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72810.4527714763297714No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45960.28580383260700853No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.2813264879708674No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.20558474120948No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.19457793564563308No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30740.19115774182635864No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24510.15241627365530416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23060.1433993990408533No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.12039082243846139No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19210.11945804230593197No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18120.11267984000955167No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC17880.11118739179750463No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17350.1078915686625674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3500.061.4090461
GTATCAA210250.048.784861
TAACGCA3450.046.5630154
TCACGCA3850.043.270684
TATCAAC260550.039.3932152
ATCAACG264550.038.727713
AACGCAG281900.038.3069886
TATCACG4350.038.299422
TCAACGC271450.038.291254
CAACGCA275000.037.9483875
AAACGCA6600.037.8618435
GTACAAC4750.036.45041
GGTTTCA9200.035.6921581
AGATTAC8650.035.0814482
ATCACGC4750.035.0720253
ACGCAGA310150.034.836987
CGCAGAG313850.034.577948
ACAACGC4850.034.3488923
GTATCAC5600.034.116141
GCAGAGT324800.033.3938949