FastQCFastQC Report
Fri 10 Feb 2017
SRR3559792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12279506
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3162032.5750465857502736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1538201.2526562550643323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT972970.792352721681149No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA592540.48254384174737974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT549130.4471922567569086No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT479420.3904228720601627No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413770.3369598092952599No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294130.23952917975690555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255200.2078259500015717No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG242850.19776854215470882No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG232370.18923399687251263No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT221630.1804877166882772No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215490.17548751554012026No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC177100.14422404288902177No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT171230.13944372029298246No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG156530.12747255467768817No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC154910.12615328336498227No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC143160.11658449452282528No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC127260.10363609089811918No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC124870.10168975852937406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG24050.048.4056241
GTATCAA1846850.041.4800261
TAACGCA24600.040.3894424
TACAACG26650.037.7295232
TATCACG22800.037.0548742
TATCAAC2221000.034.438892
ATCAACG2266400.033.66463
TCAACGC2301850.033.6475754
AACGCAG2378900.033.2180256
CAACGCA2334950.033.211365
TCACGCA26550.032.7171024
GACCGTT25850.031.9928677
ACGCAGA2568300.030.8086137
CGCAGAG2593600.030.5857058
GTATAAC36900.030.4163991
GCAGAGT2643900.029.9271819
TACTGGT88600.029.3453672
AGTACTT1422100.028.76719912-13
GTACTGG101000.028.6088451
CATGGGT177500.028.524484
Produced by FastQC (version 0.11.5)