FastQCFastQC Report
Fri 10 Feb 2017
SRR3559787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431411
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT682561.9891525672675177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313800.9144926095999576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216580.6311689273013346No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA173570.5058269032768152No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT143940.41947758516831707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117280.34178359864207464No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100190.29197901388087877No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70280.2048137049161409No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66760.19455553415198587No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG66700.19438067896850594No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT65560.19105843048238758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.1527068602391261No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50110.14603322073630934No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49880.14536294253296966No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG45920.13382250042329524No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC38680.11272330828338546No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC38030.11082904379568638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6550.049.9577522
CGCATAG7150.048.426211
TACAACG7250.042.6723752
TCACGCA8400.042.4959074
TAACGCA7000.041.6459924
ATCACGC10250.034.2454833
GTATCAA520550.032.69591
TAGTACT11600.032.3115465
GTATCAC11700.032.1449851
CATGGGT51350.029.080944
CATGGGG107600.028.7518774
TATCAAC601450.028.1426032
GTACAAC13050.027.904731
TCAACGC623700.027.6628824
ATAGTAC12700.027.6390744
GTATAAC11700.027.5528431
ATCAACG614050.027.535663
CAACGCA634050.027.3051555
AGTACTT317050.027.26666512-13
AACGCAG661000.026.8309276
Produced by FastQC (version 0.11.5)