FastQCFastQC Report
Fri 10 Feb 2017
SRR3559767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6700175
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1497682.235284899275019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT707021.055226169465723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471100.7031159633890159No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236820.35345345457394767No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166840.24900842142182855No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119580.1784729503333868No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113510.16941348546866314No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106560.15904062207330405No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97020.14480218800255218No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT91680.13683224691892376No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC83260.12426541097807146No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81920.12226546321551301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA634850.058.879541
TATCAAC814850.045.867572
ATCAACG826650.045.1120723
TCAACGC840000.044.834244
CAACGCA849600.044.362665
AACGCAG870650.043.836766
ACGCAGA955400.039.9057667
CGCAGAG969050.039.4111948
CGCATAG9500.038.9636761
GCAGAGT1010550.037.7456059
GGTATCA320700.035.557091
TAACGCA11800.032.7730454
CATGGGT115050.032.527414
GTACTGG52950.031.5706751
CATGGGG227650.029.924254
AGTACTT639650.029.49525812-13
AGAGTAC993500.029.44629910-11
TATCACG11400.028.1821632
ACATGGG615550.028.1747883
GAGTACT588500.027.96430812-13
Produced by FastQC (version 0.11.5)