FastQCFastQC Report
Fri 10 Feb 2017
SRR3559761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464019
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT757703.075057456943311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325081.319308008582726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258581.04942372603458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126180.5120902070966173No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90230.3661903581100633No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66060.2680985820320379No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49320.200160794214655No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.1926527352264735No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33270.13502330947935062No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26310.10677677404273263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA267150.064.2587661
CGCATAG7250.058.434321
TAACGCA5600.050.998394
TATCAAC343950.049.749422
ATCAACG347750.049.189683
TCAACGC357000.048.715134
CAACGCA363500.047.909495
AACGCAG374450.047.5095186
TACAACG6100.046.817242
GGTATCA122150.045.9667551
ACAACGC6250.044.7425883
ACGCAGA418750.042.440817
CGCAGAG423750.042.0127948
ATAGTAC10150.041.6193774
GTATAAC8050.041.5087661
GCAGAGT438150.040.4554829
TATCACG4050.038.195552
ATAACGC9400.037.9775243
TAGTACT11200.036.6550945
GTACAAC8850.035.733891