FastQCFastQC Report
Fri 10 Feb 2017
SRR3559759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5071280
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1686163.3249199413165904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT717531.4148893376031297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508051.001818081431118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284170.5603516272025998No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185910.36659383824202174No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150960.2976763262923759No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129490.25533987474562636No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116220.2291729109810541No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74630.14716205770535248No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC63410.12503746588632456No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59900.11811613636005111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA584550.065.542481
CGCATAG10650.052.664761
TATCAAC747700.051.237672
ATCAACG762100.050.0514223
TCAACGC780750.049.457864
TATCACG8950.049.193422
CAACGCA789100.049.055155
AACGCAG806300.048.739246
GGTATCA264600.044.604461
ACGCAGA895350.043.9116557
CGCAGAG905800.043.426478
GCAGAGT943900.041.6294639
TACAACG9600.039.04522
TCACGCA11250.038.0787434
CATGGGT92250.036.7630124
TAACGCA10550.034.4017034
GTATCAC15800.032.855191
ATCACGC13600.032.373933
AGATTAC21100.032.145542
TAGTACT19700.031.1081075