FastQCFastQC Report
Fri 10 Feb 2017
SRR3559758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971124
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251762.5924598712419833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122911.2656468175021933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103021.0608326022217553No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49890.5137346003188058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.5037461745358986No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.31829097005119844No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22060.22715945646487987No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.1733043360065244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.17206865446637093No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.1622861756068226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12300.12665735786573085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12200.12562762324893628No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10700.11018160399701789No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10030.10328238206449435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2700.066.2815251
TACAACG2500.054.7369962
TAACGCA2950.054.454634
GTATCAA117550.049.3770561
TATCACG1950.048.817832
ACAACGC2700.048.4788173
GGTTTCA5550.048.36761
ACGCATA1004.0527084E-947.72271
AGATTAC5450.045.8506932
GTACAAC3350.040.9560471
GTATAAC3950.040.7757231
TATCAAC144600.040.2816732
ATCAACG145450.039.9235573
TCAACGC149200.039.797414
GATTACT6300.039.664493
CAACGCA151350.039.585875
AACGCAG156850.039.5254026
ATCACGC2800.038.24793
CATGGGT13300.036.6821944
GGTATCA68150.036.5010381
Produced by FastQC (version 0.11.5)