FastQCFastQC Report
Fri 10 Feb 2017
SRR3559757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075969
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509222.4529268018934776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236331.1384081361523222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193520.9321911839724004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93430.4500548900296681No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.34764488294382045No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.23733495057007112No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35350.1702819261751982No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.1692703503761376No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT32310.15563816222689258No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.1384413736428627No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT24730.1191250929084201No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC23980.11551232219748947No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22920.1104062729260408No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA21480.10346975316105395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA212500.053.419511
TAACGCA4050.051.417674
CGCATAG4700.050.767081
TATCAAC269600.041.9749342
ATCAACG273900.041.207353
TCAACGC281700.040.8689544
CAACGCA284450.040.557515
GGTATCA117550.040.291921
AACGCAG293400.040.0300756
CATGGGT35450.039.105614
TATCACG3350.039.0730442
TACAACG4700.037.9771842
TACTGGT13900.036.811462
GTACTGG15950.036.6509671
ACGCAGA326700.035.9316677
GGTTCAC13700.035.61176
CGCAGAG330850.035.610278
ACAACGC5150.034.6587943
GCAGAGT341050.034.5103579
GTATAAC6400.034.4859161