FastQCFastQC Report
Fri 10 Feb 2017
SRR3559756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3528454
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT891152.525610366466447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410911.164561023042953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330980.9380312170712726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155340.44024946903091267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101570.2878597822162341No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75160.21301113745566758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61490.1742689574527541No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52470.14870535367614257No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.14198853095435No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43990.12467216520323066No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36190.10256616637201448No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.10072399980274648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA371300.057.1855851
CGCATAG8650.052.418071
TATCACG6200.046.06342
TATCAAC464900.045.6893652
TAACGCA7800.045.0053254
ATCAACG474700.044.7342263
TCAACGC486550.044.4389654
CAACGCA493000.044.0989345
AACGCAG507700.043.8299456
TACAACG8050.042.8685262
GGTATCA193200.040.94681
ACGCAGA559050.039.7827767
CGCAGAG564350.039.473548
GTATAAC9600.039.15191
GCAGAGT590050.037.7340779
ACAACGC9450.036.518153
GGTTTCA19100.032.79741
AGATTAC17400.032.4848482
ATAACGC12700.032.326453
TCACGCA9050.031.5572474