FastQCFastQC Report
Fri 10 Feb 2017
SRR3559753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225456
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1133193.513270681726863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488151.5134294189720772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364031.1286156128001748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196750.6099912694515132No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130600.40490398876933986No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101100.3134440525618703No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83020.25738996284556354No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77540.24040011706871833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA65110.20186293038875744No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49040.15204051768184096No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC39790.12336240209136322No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37770.11709972171376698No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35680.1106200177587293No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT32640.10119499382412905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8300.063.9866751
GTATCAA415950.061.7459871
TACAACG7150.049.9277342
TATCAAC524050.048.8420942
ATCAACG533850.047.7909433
TCAACGC548050.047.258354
CAACGCA557250.046.542925
AACGCAG568500.046.417316
TAACGCA6100.043.8927464
TATCACG6200.043.183462
GCATAGT12500.042.8379942
ATAGTAC12650.042.8016934
ACGCAGA629250.041.8981977
CGCAGAG635200.041.470198
TAGTACT13300.041.157885
GCAGAGT663800.039.602769
GGTATCA200950.039.3761441
AGATTAC15000.039.268162
GGTTTCA17950.037.5657121
GTACAAC11500.035.2848851
Produced by FastQC (version 0.11.5)