FastQCFastQC Report
Fri 10 Feb 2017
SRR3559751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203058
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT859942.684746888754434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366961.145655183265492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282630.8823755298842544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143370.4476035088968105No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81370.2540384844732752No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66700.20823850208144842No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60880.19006836591781978No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56610.17673735536477955No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43060.1344340314786682No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC35410.11055060507802232No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT32940.10283922426631051No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32280.10077869336115675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA325550.063.591991
CGCATAG6450.058.2735521
TATCAAC409250.050.434182
ATCAACG415150.049.6329843
TCAACGC424900.048.9834374
CAACGCA428800.048.6204225
AACGCAG439750.048.370396
GGTATCA151050.045.5406531
ACGCAGA488400.043.5887157
CGCAGAG495750.042.9558148
TATCACG5550.041.810082
GCAGAGT516900.041.1406339
ATAGTAC10650.035.1971174
TAGTACT11250.034.906065
TAACGCA6350.034.6692244
TCACGCA6700.034.6342664
AGAGTAC510150.030.45217910-11
CATGGGG96900.030.2718754
AGATTAC15650.030.034622
GGACCGT11050.029.6148686
Produced by FastQC (version 0.11.5)