FastQCFastQC Report
Fri 10 Feb 2017
SRR3559750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3031906
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT852672.8123233372010876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404261.333352683097695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309031.0192598319341035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153770.5071727157768084No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111600.36808529024316716No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80450.26534463799339425No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58570.19317881227188444No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.1792931575055427No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45410.14977377266973319No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.12358562567573006No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT34670.11435051086676169No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC32390.10683048880803032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31310.10326837309599966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA339000.057.225121
TAACGCA6950.052.2221764
CGCATAG9400.049.4997061
TACAACG6150.048.37242
TATCAAC427950.045.3378072
GTATAAC8250.044.8305051
ATCAACG432600.044.7686923
TCAACGC444700.044.4336174
CAACGCA449350.044.1062165
ATAGTAC10800.044.073364
AACGCAG460950.043.9256326
GGTATCA176350.041.84371
ACGCAGA506650.039.9635287
CGCAGAG515000.039.4690258
TAGTACT12400.038.8663065
TATCACG5950.037.998762
GCAGAGT534350.037.939549
ACAACGC8650.035.7682083
ATAACGC12250.034.4851533
AGTGTAC13900.033.3879473
Produced by FastQC (version 0.11.5)