FastQCFastQC Report
Fri 10 Feb 2017
SRR3559749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581110
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1078824.179674636106171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471531.8268496886998231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361541.400715196175289No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171580.6647527614088512No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105280.40788652943888487No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84710.3281921343917927No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.2850324085374122No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.25717617614127253No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49660.19239784433828858No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48430.1876324527044566No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38130.14772714064879064No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT36870.14284551995071887No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA36080.1397848212590707No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC35190.13633669235328985No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.11529923172588538No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT28340.10979772268520133No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27650.10712445420768585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA352250.066.965121
CGCATAG6150.065.979131
GGTATCA156050.051.890621
TATCAAC456900.051.684522
ATCAACG469500.050.1580623
TAACGCA5600.049.9356164
TCAACGC481550.049.6566244
CAACGCA489250.048.9480745
AACGCAG500050.048.62866
TACAACG4900.043.7126352
ACGCAGA558750.043.5837677
CGCAGAG562900.043.338118
GTATAAC7750.043.118051
GCAGAGT584400.041.672449
ATAGTAC10550.040.041144
ATAACGC7550.039.402483
AGATTAC12700.039.3528442
AGTGTAC11150.038.9536863
GACCGTT5400.038.563367
GTATCAC7600.037.687771