FastQCFastQC Report
Fri 10 Feb 2017
SRR3559742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739570
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130952.3861869325698324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476921.006251621982585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378100.7977516947739984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150150.3168008912200896No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75620.1595503389547997No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.12138231949311858No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55370.1168249440350074No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53920.11376559476914572No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.11102272991009732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA438900.067.4104461
TATCAAC571450.051.6843832
ATCAACG581750.050.575513
GGTATCA190100.050.52921
TCAACGC592100.050.26374
CAACGCA598200.049.830715
AACGCAG614050.049.213056
ACGCAGA678050.044.6030127
CGCAGAG688950.044.0450828
GCAGAGT722050.041.9353379
CGCATAG7600.038.4663621
AGAGTAC709850.032.02314810-11
TACAACG9650.031.444792
AGTACTT523000.029.99983212-13
TAACGCA8650.028.8894314
GAGTACT487250.028.77570512-13
CAGAGTA717050.028.2165410-11
CATGGGG146200.027.5516174
TATCACG7600.027.4006122
ACATGGG374600.027.0493773
Produced by FastQC (version 0.11.5)