FastQCFastQC Report
Fri 10 Feb 2017
SRR3559740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993593
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT769472.5703894951651747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318911.0653084771376737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267190.892539500192578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108570.362674551951451No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77420.25861899062431No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62280.2080443133051153No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.16458483167217455No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46980.15693516119258696No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.15112274781508375No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44100.14731461491258163No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41620.13903025561591037No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.13107994306507262No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.1247998642434025No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC34430.1150122945904804No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.10448982209672457No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30860.10308682576422379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8150.061.476141
GTATCAA409300.053.598781
TACAACG7750.043.7598572
TATCAAC502850.043.4713822
ATCAACG514350.042.4994353
TATCACG5900.042.3545462
TCAACGC525550.042.306964
CAACGCA531700.042.0414125
AACGCAG544800.041.969726
AGATTAC15000.040.458682
TAACGCA6850.039.95494
GCATAGT13100.039.059792
ACGCAGA598450.038.2072077
CGCAGAG605350.037.8823558
ATAGTAC13600.037.1862834
GCAGAGT621200.036.77219
GTACAAC10200.036.2557141
ACAACGC9100.035.3065383
TCACGCA7500.034.9055254
AGTGTAC16600.034.7669373