FastQCFastQC Report
Fri 10 Feb 2017
SRR3559739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11718690
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2295761.9590585637131797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT996180.8500779523991163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT802410.6847267058007337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294410.2512311529701699No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT181390.15478692584239365No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC169000.1442140717093805No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152450.13009133273429027No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA136640.11660006365899261No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT126720.10813495365096269No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC121910.1040303993023111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA881300.074.1960451
GGTATCA333050.065.498341
TATCAAC1188150.055.0383722
ATCAACG1206850.054.0531433
TCAACGC1222350.053.489414
CAACGCA1235850.052.895485
AACGCAG1260550.052.0006186
ACGCAGA1408750.046.466827
CGCAGAG1427450.045.8725558
GCAGAGT1493200.043.721159
AGAGTAC1456750.033.56393410-11
GTACTGG101100.032.2738571
TACTGGT84050.031.7839812
CATGGGT167900.030.6528344
AGTACTT1042450.029.87422812-13
CAGAGTA1475150.029.56151810-11
GAGTACT939200.029.41431812-13
GTACATG867700.027.807611
TACTTTT1122450.027.76827214-15
TACATGG876300.027.4708922