FastQCFastQC Report
Fri 10 Feb 2017
SRR3559738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4962660
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT991681.9982831787791224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432570.8716494783039741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351580.7084507099015447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143460.2890788407829672No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72260.14560739603357878No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66670.13434327558204673No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55180.11119036968077604No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT54950.11072690855307436No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54590.11000149113580217No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC53710.10822824856024794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA470800.057.951021
TATCAAC597100.045.557142
ATCAACG604900.044.86243
TCAACGC619050.044.5770234
CAACGCA624050.044.2961355
AACGCAG642350.043.932656
CGCATAG10750.043.8348961
TAACGCA10050.042.033774
ACGCAGA711950.039.6461687
CGCAGAG721200.039.199378
GGTATCA239150.038.5852471
GCAGAGT752300.037.499789
ACAACGC10500.035.6991043
TATCACG8200.033.3765372
GTATAAC16350.032.4693531
AGAGTAC739000.028.85789310-11
AGTACTT501650.028.57439612-13
TACAACG12750.028.4653762
GTACAAC16650.027.943561
GAGTACT457250.027.65325712-13