FastQCFastQC Report
Fri 10 Feb 2017
SRR3559731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125312
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1093523.498914668359511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485441.553252923228145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357051.1424459381975303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187560.600132082812852No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126220.40386367825036346No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96870.30995305428706No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.2475592836811173No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.2275932770872156No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47840.15307271721991278No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35570.11381263694632729No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.11234078389613582No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC32640.10443757295271641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9450.064.393691
GTATCAA379250.062.230841
TAACGCA6800.051.6228834
TATCAAC482800.048.9733432
ATCAACG492450.047.9411773
TCAACGC503850.047.4823274
CAACGCA508700.047.0179255
AACGCAG519950.046.8588336
ATAGTAC13450.046.0055474
TAGTACT13850.045.9656225
GGTATCA179500.044.270531
TATCACG6450.042.432372
ACGCAGA575700.042.279757
CGCAGAG582300.041.9551438
TCACGCA6800.040.248354
GCAGAGT607850.040.1328969
GCATAGT15550.039.792582
AGATTAC16100.039.1723062
GTATAAC10300.035.911161
CATAGTA18250.034.2314723
Produced by FastQC (version 0.11.5)