FastQCFastQC Report
Fri 10 Feb 2017
SRR3559729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4695040
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1729663.684015471646674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT755071.6082291098691384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541391.1531105166303162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281920.6004634678298801No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174760.37222260087241005No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139820.29780363958560524No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123300.26261757088331517No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116790.24875187431842966No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77420.16489742366412213No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62070.13220334651035986No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC53080.113055479825518No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT48340.10295971919302072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA589850.065.911711
TATCAAC752450.051.641582
ATCAACG773850.050.167893
CGCATAG9400.049.517251
TCAACGC793000.049.4515844
CAACGCA803100.048.8889355
AACGCAG820300.048.6254236
TACAACG8300.048.027962
TATCACG8300.045.8774532
GGTATCA263150.045.6715621
ACGCAGA912650.043.7572257
CGCAGAG922550.043.3548
GCAGAGT958300.041.6497279
TAACGCA8450.038.0223664
ACAACGC10500.037.9653663
TCACGCA11200.035.0612954
CATGGGT89000.033.6263734
TAGTACT15150.032.9889685
AAACGCA15450.032.3484085
AGATTAC20150.031.2988412