FastQCFastQC Report
Fri 10 Feb 2017
SRR3559728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291053
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT742892.2573018422978905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324690.986583929216576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254110.7721236941489548No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128240.3896625183489904No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79780.24241481373894613No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA65650.19948022714918295No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61590.18714375003988085No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52200.15861184854816984No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48230.14654884014326114No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46160.14025905994221302No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38090.11573803278160516No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37810.11488724125682571No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33690.10236845167792802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA338550.054.6608121
CGCATAG7200.045.5708661
TATCAAC418400.044.3240432
ATCAACG424600.043.5653883
TCAACGC432950.043.4260374
CAACGCA436600.043.1992725
AACGCAG446600.042.964716
TACAACG6900.039.6645932
ACGCAGA489300.039.1666457
CGCAGAG495600.038.7065478
TATCACG5700.037.5769842
GCAGAGT513450.037.2450339
ACAACGC7350.037.236723
TAACGCA5350.035.5874674
GGTATCA183600.031.8102551
ATAGTAC10450.031.314634
CATGGGG109150.030.68924
TAGTACT11700.030.0031645
GTACTGG24600.029.58571
AGTACTT327400.028.49583812-13
Produced by FastQC (version 0.11.5)