FastQCFastQC Report
Fri 10 Feb 2017
SRR3559726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4148072
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1167752.815163285497455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531471.2812458414415178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386540.9318546061881279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201290.4852615865876967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131150.31617098256732284No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97790.23574807766113992No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80260.19348748044874822No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74380.1793122202314714No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72110.17383979834486962No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55450.13367656106258521No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT50170.1209477559695203No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC45250.10908682395098251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA465000.057.0650251
CGCATAG10850.046.735551
TATCAAC588300.045.0561262
ATCAACG598000.044.1760373
TCAACGC612300.043.649094
CAACGCA619900.043.35395
AACGCAG633800.043.154096
TATCACG6450.040.5880282
TAACGCA6950.040.2358134
ACGCAGA698800.039.1145067
CGCAGAG708150.038.6671378
GCAGAGT737000.037.0889179
GGTATCA243800.035.8967061
TACAACG11000.034.6172262
ACAACGC11150.033.6179053
AGATTAC17900.030.9125522
ATAGTAC16750.030.5480424
GTACAAC15200.029.8282781
GTATAAC10600.029.8282781
TAGTACT17550.029.4945555