FastQCFastQC Report
Fri 10 Feb 2017
SRR3559721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812304
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1153024.099912385005319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT513011.8241626794258374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383601.3640061671853398No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184960.6576813886407729No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100310.35668263459426863No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84970.3021366111202772No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83120.29555837491252723No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70110.2492973732569452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43350.15414407546268113No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43180.15353958889223923No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT37730.13416046060454348No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32280.11478133231684767No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA29600.10525177932399911No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.10475396685422345No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC28850.10258492680734373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA399100.067.898721
CGCATAG6200.065.4499051
TATCAAC515750.052.5462652
ATCAACG528100.051.262013
TCAACGC541350.050.5568664
CAACGCA549450.049.9306645
GGTATCA171650.049.818911
AACGCAG560750.049.5398866
TACAACG6200.048.9408152
ACGCAGA623700.044.4730537
CGCAGAG627300.044.202798
TAACGCA5600.043.560844
GCAGAGT651750.042.462389
ATAGTAC10100.040.057884
AGATTAC12700.039.820612
GTACAAC9300.038.4999471
TAGTACT11000.037.3213045
GTATAAC7050.037.243921
GCATAGT11600.036.416072
CATGGGT47350.035.6860734
Produced by FastQC (version 0.11.5)