FastQCFastQC Report
Fri 10 Feb 2017
SRR3559719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473683
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472213.204284774948208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236361.6038727460383273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174871.1866188318654691No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86450.5866254818709316No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76250.5174111393020072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.32605383925851084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.24150376980666804No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.20336802419516276No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26830.18206086383570957No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.16245013344118106No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16340.11087866250747277No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC15580.10572151541410194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5250.066.992331
TATCACG2800.061.6234932
GTATCAA186350.054.7654531
TAACGCA4550.053.6086164
ATAGTAC6950.050.504414
AGATTAC9550.049.2186852
TACAACG3300.048.6806222
TAGTACT7550.048.0667765
GCATAGT8000.045.3676342
ATAACGC6200.045.1037333
GTATAAC6100.044.9531751
TCACGCA4000.044.619364
GATTACT10650.044.1350673
TATCAAC232950.043.828932
ATCAACG237200.043.0436333
TCAACGC243700.042.794554
AACGCAG255800.042.4680526
CAACGCA248350.042.2567945
GTATCAC5000.040.5360371
GGTATCA100750.039.7609371