FastQCFastQC Report
Fri 10 Feb 2017
SRR3559717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310357
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269062.053333557190903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126310.9639357823860215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99260.7575034895070579No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.4572799626361365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.35776509760317227No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36370.27755794794853617No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.24321616170249788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21000.16026166914817871No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18750.14309077602515954No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.1384355561118077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17840.13614610369540514No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.13568821321212463No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17670.13484874732611038No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.11370947001466013No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT14890.11363315493411337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3850.068.1492841
TAACGCA3800.064.191014
TACAACG2750.060.5781252
GTATAAC4700.052.0182041
ATAGTAC5800.045.133464
GTATCAA166400.041.103561
TAGTACT6600.040.5641635
GCATAGT6950.037.666772
GTACAAC4450.037.5203931
AGATTAC6900.037.0774542
TATCACG2100.036.831092
ACAACGC4600.036.2151833
CATGGGT22650.034.6720624
ATAACGC9000.033.714613
TATCAAC204000.033.5687872
TCAACGC209100.033.46014
ATCAACG205250.033.4513133
CAACGCA211950.033.2628065
AACGCAG217750.033.2238046
GATTACT7750.033.010893