FastQCFastQC Report
Fri 10 Feb 2017
SRR3559713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203826
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295832.4574149420265057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142461.1833936133627285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112550.934935779755546No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63080.5239959927763647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54830.455464494038175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.28384500750108405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25210.20941564644724403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.1771850749194651No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.16347877517182716No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.15434124200673519No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14120.11729269844645322No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13290.11039801433097474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2900.071.807232
CGCATAG4100.066.878221
TAACGCA4450.057.4895024
GTATAAC4350.056.1830521
TATCACG2550.053.6643452
ATAACGC5200.052.6323363
GTATCAA143850.050.6789131
GTACAAC4550.049.783211
AGATTAC8800.046.6513942
ACAACGC4350.046.5037353
AAACGCA4700.043.0371135
GTATCAC4450.042.8647881
GATTACT9700.042.322913
TATCAAC177700.040.9819072
ATAGTAC6900.040.5255474
ATCAACG180600.040.2579463
TACGCAG3700.040.1976245
TCAACGC185700.040.047764
AACGCAG196150.039.9144026
CAACGCA188550.039.724765