FastQCFastQC Report
Fri 10 Feb 2017
SRR3559708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890343
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415712.199124709113637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195661.035050252784812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149290.7897508547390606No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76160.4028898459168521No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74980.3966475925268589No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43700.23117497723958033No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34540.18271816278844633No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.15700854289406738No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25370.13420844788485475No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.1191847193869049No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19980.1056951040102246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4050.055.8223232
TAACGCA5100.053.6620184
CGCATAG6550.052.8011741
GTATCAA201250.050.9919781
AGATTAC11300.044.2262542
AAACGCA6900.043.1110655
TATCACG3750.041.2497562
TATCAAC255750.040.1284872
GTATAAC7150.040.0305981
ACAACGC5800.040.0051543
GATTACT12500.039.9805343
ATCAACG257600.039.7941063
TCAACGC264100.039.580634
AACGCAG277600.039.5407376
ATAGTAC8950.039.2200624
CAACGCA269550.039.0883265
TAGTACT9050.038.785675
ACGCATA3000.037.7649771
GCATAGT9450.037.1449242
GTACAAC7100.036.113281