FastQCFastQC Report
Fri 10 Feb 2017
SRR3559702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3821034
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1229703.218238832734804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT578541.5140927822154946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409311.071202192914274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209990.5495632857493548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138390.3621794519493938No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104180.27264871236424487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95560.25008937371402606No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.22043771398003786No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77660.2032434152640359No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60900.15938094243600032No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC60160.15744429387438058No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51440.1346232459590781No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49070.12842073637659335No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45810.11988901433486329No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43620.11415758142952928No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC40670.10643715810955882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG12650.065.516561
GTATCAA519950.055.4449161
TACAACG9150.053.9690932
TATCACG8550.047.3184932
AGATTAC22350.045.5204662
ATAGTAC18500.045.023454
TAACGCA9250.044.3802574
TATCAAC652850.044.153842
GTATAAC11100.044.047141
ATCAACG665850.043.2203033
TCACGCA10400.042.9052434
GGTTTCA20750.042.814911
TCAACGC688050.042.698594
AGTGTAC19800.042.6688843
TAGTACT19950.042.347515
AACGCAG713700.042.331096
CAACGCA698450.042.2842755
ACAACGC12250.040.7972873
GTACAAC13900.040.7507551
GTATCAC13400.039.601541