FastQCFastQC Report
Fri 10 Feb 2017
SRR3559701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269965
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT869383.8299268931459296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394901.7396744002660836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300701.3246900282603475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129490.5704493241085214No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69410.3057756397125066No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51170.2254219778719055No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44160.19454044445619206No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.19392369485873132No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.1855094682076596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41450.1826019343910589No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC36570.16110380556528403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32580.14352644203765255No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31240.13762326731909963No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.12255695572398694No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC27220.11991374316344085No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA27110.11942915419400739No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26570.11705026288951592No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT23850.10506769928170698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG5350.076.738562
CGCATAG9900.072.261521
GTATCAA340400.063.5212551
ATAGTAC12550.057.364194
TAGTACT13350.054.371115
AGATTAC17350.054.184612
GCATAGT13700.052.5512582
TAACGCA7100.051.955624
ACAACGC8350.049.8803943
TATCAAC432350.049.8323552
CATAGTA14400.049.170293
GGTTTCA14700.049.071471
AGTGTAC12550.048.832823
GTACAAC8800.048.7765241
ATCAACG444950.048.4078373
TATCACG5600.047.812652
TCAACGC464850.047.74144
GTATAAC7250.047.69261
AACGCAG484550.047.604376
CAACGCA472400.047.4055675
Produced by FastQC (version 0.11.5)