FastQCFastQC Report
Fri 10 Feb 2017
SRR3559700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6555197
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1493892.2789399006620243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT699491.0670770077543055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494490.7543480386630638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208770.3184801311081879No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114480.17464006039787972No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.1316817785949072No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA86120.13137667716164747No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77590.11836410103311922No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74990.1143977824007425No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69170.10551933069288383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA619100.061.685721
CGCATAG14900.057.614891
TATCACG11100.047.169292
TATCAAC817800.046.8167342
ATCAACG826050.046.327553
TCAACGC847450.046.026154
CAACGCA858400.045.611955
AACGCAG877350.045.433736
GGTATCA298050.043.2639241
TAACGCA12650.042.328394
TACAACG12450.041.0987632
ACGCAGA972500.040.902827
CGCAGAG988350.040.4093978
GCAGAGT1035850.038.464489
ATAGTAC23950.036.516714
TAGTACT26250.033.7701845
TCACGCA15750.033.24164
AGATTAC28750.032.490882
ACAACGC16000.031.2362563
AGAGTAC1017750.030.53504810-11