FastQCFastQC Report
Fri 10 Feb 2017
SRR3559695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173515
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1132503.5685982262570053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529681.6690641134514885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364261.147812441409604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196790.6201010551391754No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144660.45583524892745114No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104080.3279644180033811No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88670.27940627348539393No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76720.24175086615314564No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA71260.22454596874443636No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55220.17400264375621355No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52620.16580983546635197No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43920.1383954384964306No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39640.1249088156192739No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37560.11835456898738464No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT37100.11690507213610146No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.1160857913071153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11850.068.422631
TACAACG8250.060.575962
TATCACG6900.059.4942472
GTATCAA448800.055.101711
TCACGCA8100.052.148464
TAACGCA7900.050.4563524
AGATTAC20600.047.6531642
TAGTACT18400.045.911875
ACAACGC11150.045.354363
ATAGTAC18450.044.8215184
GGTTTCA19050.044.126891
GTACAAC11950.043.9029731
TATCAAC566300.043.525462
GCATAGT18850.042.9242322
ATCAACG579150.042.5700043
TCAACGC598650.041.9976164
AACGCAG627450.041.879626
CAACGCA608450.041.681655
AAACGCA15200.041.487455
GTATCAC11500.040.9551431