FastQCFastQC Report
Fri 10 Feb 2017
SRR3559694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6537750
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2131083.2596535505334403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1027111.5710450843179993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671281.026775266720202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362040.5537684983365837No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235660.3604604030438606No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176490.2699552598370999No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168420.25761156361133414No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA155990.23859890635157355No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143730.21984627738901No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT119270.1824327941570112No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC87420.13371572788803487No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83930.12837749990440137No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT82960.12689380903215938No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG80450.12305456770295592No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC73640.11263814003288593No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC70620.1080188138120913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG19800.059.3169941
TATCACG12150.054.8468932
GTATCAA856750.054.5139431
TACAACG16900.051.753642
TAACGCA15500.045.6767964
AGATTAC33900.043.5273552
TATCAAC1087100.042.866052
ATCAACG1112850.041.874183
TCAACGC1142850.041.584284
TCACGCA16350.041.4827464
AACGCAG1193150.041.1575786
CAACGCA1163350.041.1276665
ATAGTAC29400.039.6633264
GTACAAC24350.038.6844331
AGTGTAC32700.037.846513
ACGCAGA1306950.037.5920757
ACAACGC23450.037.297933
CGCAGAG1322450.037.2864388
GTATCAC22400.037.261311
GTATAAC21950.036.9387741