FastQCFastQC Report
Fri 10 Feb 2017
SRR3559689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6116336
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280682.093867962780331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596050.9745213474210704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399960.6539209095118385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206470.33757138260553377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161970.2648154058246637No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117340.19184688349364717No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103590.16936610415124348No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84480.13812190827972826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA481950.062.9649281
TATCAAC624500.048.474422
ATCAACG626950.048.0572283
TCAACGC640450.047.8694734
CAACGCA651600.047.2687075
AACGCAG673000.046.826486
TAACGCA12900.044.275684
CGCATAG13050.043.8574751
ACGCAGA745950.042.2231757
CGCAGAG760850.041.497958
TATCACG9400.039.875912
GCAGAGT797700.039.528749
GGTATCA226950.039.2729871
TACAACG14200.034.7766462
GTATAAC20050.033.3032461
ACAACGC16400.030.4742763
AGAGTAC783500.030.3435710-11
AGTACTT573100.028.57982312-13
AGATTAC28650.028.4507622
CATGGGG177750.028.4172334
Produced by FastQC (version 0.11.5)