FastQCFastQC Report
Fri 10 Feb 2017
SRR3559685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284251
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1291002.054341877814874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT605590.9636629727234001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389340.619548773592907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220780.35132269541748096No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA195020.31033133463319656No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT149290.23756212156389045No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148070.23562076053295772No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109180.17373589947314327No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103590.16484064688059086No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT102850.16366310002576281No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88020.14006442454319534No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78580.12504274574647No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG73080.11629070831193726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG14250.047.2740971
GTATCAA694050.046.7785261
TATCACG9050.046.021232
TACAACG12450.040.1438182
TATCAAC847850.038.1407552
ATCAACG866650.037.2378583
TCAACGC883900.037.1151854
CAACGCA898300.036.638565
AACGCAG923000.036.5540586
ACGCAGA1006150.033.5449947
CGCAGAG1020750.033.1701058
CATGGGT94600.032.5152744
TAACGCA14300.032.452384
GCAGAGT1049350.032.2433749
AGATTAC29350.032.030062
ACAACGC17250.030.0081273
ACGCATA6950.029.1644521
TAGTACT24150.029.0698345
AGTACTT597500.028.64227912-13
ATAGTAC24500.028.4123944
Produced by FastQC (version 0.11.5)