FastQCFastQC Report
Fri 10 Feb 2017
SRR3559681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520698
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1019442.895562186816364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462141.3126374372354572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315590.8963847509783571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166260.4722359032214634No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112540.3196525234484753No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83400.2368848449938052No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79720.22643237221710014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68260.19388200862442617No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.1922062045651175No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49400.14031308564381267No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.11622695272357925No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40110.11392627257435883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA395600.059.402611
CGCATAG9600.057.7506561
TATCACG5950.054.995422
TACAACG6750.053.7658922
TATCAAC506550.046.181922
ATCAACG517600.045.069563
TCAACGC533100.044.7728274
AACGCAG557900.044.4122126
CAACGCA542050.044.2841155
ACGCAGA613600.040.3806657
CGCAGAG623450.039.876278
ACAACGC9750.039.0531543
GCAGAGT649800.038.1951649
TAACGCA9150.037.7110984
TAGTACT15700.036.3760345
AGATTAC17300.036.1097072
ATAGTAC15800.035.394284
GGTATCA196600.035.3254431
TCACGCA9050.034.8409164
GTATCAC13350.033.937321