FastQCFastQC Report
Fri 10 Feb 2017
SRR3559680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9453861
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2036942.154611750691067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT986781.043785179409767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624150.6602064489841769No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338250.3577903250322805No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214610.22700778020747292No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171380.18128043134968877No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168780.17853023225114056No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA146000.15443425707232208No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143230.1515042372634842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT123450.1305815687368367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA843650.059.337621
TATCAAC1099250.045.513482
ATCAACG1114800.044.7985763
TCAACGC1138550.044.536784
CAACGCA1154800.044.0491755
AACGCAG1183000.043.823926
CGCATAG17600.042.3414341
TATCACG15000.040.854342
ACGCAGA1303050.039.7225047
CGCAGAG1324400.039.1809848
GCAGAGT1386700.037.3434839
GGTATCA423400.034.8773461
TACAACG16550.034.1521342
CATGGGG272700.031.372724
CATGGGT108100.029.5547314
AGAGTAC1359850.029.42930610-11
AGTACTT938150.029.12142612-13
TCACGCA22450.027.5610284
ACATGGG768750.027.2736323
GTACATG827800.026.5603471