FastQCFastQC Report
Fri 10 Feb 2017
SRR3559676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726312
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487232.8223750978965563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244201.4145762758991423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155740.9021544193633596No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94780.5490316929963992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94710.5486262043014241No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61980.359031275922313No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39250.22736330396822824No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38410.222497439628526No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35080.20320776313899228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26250.15205826061569402No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22710.13155211804123473No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22700.1314941910848097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5150.064.7759551
TATCACG2750.056.2610632
GTATCAA179300.054.6868061
TAACGCA3600.042.9709784
TAGTACT8200.042.809745
TATCAAC229650.042.495672
ATCAACG230850.042.191333
TCAACGC238000.041.6237954
GCATAGT8050.041.3961032
AACGCAG248900.041.329676
CAACGCA242700.041.062885
TACAACG4100.040.6388552
ATAGTAC8400.040.3738334
GTATAAC4500.039.713831
ACGCAGA275300.037.2582867
ACAACGC5000.036.888933
CGCAGAG279300.036.7672928
AAACGCA7450.036.737195
ATCACGC4300.035.97573
GCAGAGT290650.035.2905739