FastQCFastQC Report
Fri 10 Feb 2017
SRR3559675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774045
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT741211.9639670433182437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362610.9607993545386979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223970.593448143835063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139400.3693649651766209No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116680.30916430514209553No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85050.22535502358874893No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70960.18802107553036596No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60310.15980201613918224No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.15198546917167124No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53550.14189020003736044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7550.051.28831
GTATCAA337500.049.829781
TATCACG6250.048.5564422
TAACGCA7150.042.438824
TCAACGC438100.039.1399154
ATCAACG428050.039.044193
TATCAAC428600.038.9992522
CAACGCA442800.038.8722765
AACGCAG455850.038.8036086
TACAACG7650.037.3367462
ACGCAGA498550.035.480147
CGCAGAG505150.035.122588
GCAGAGT528300.033.538479
ACAACGC9850.031.4098823
GTATAAC11050.029.6518841
GTATCAC13950.029.4663811
CATGGGG102500.029.4295274
ATCACGC10750.029.3336813
TAGTACT13650.029.2039325
ATAGTAC13550.028.9803624