FastQCFastQC Report
Fri 10 Feb 2017
SRR3559672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5402228
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1092342.022017582375272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT527190.9758751389241624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328600.6082675518323181No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190500.3526322843093627No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141390.26172534739370495No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107880.1996953849411761No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82620.1529368993681866No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77030.14258931685223208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA367700.067.889561
TATCAAC492700.050.49652
ATCAACG493700.050.2908063
TCAACGC504200.049.975134
CAACGCA510900.049.4362075
AACGCAG526250.048.909566
GGTATCA155950.047.9829371
ACGCAGA583250.044.1399157
CGCAGAG594300.043.3792768
GCAGAGT630100.040.8862959
TATCACG7300.033.4214022
AGAGTAC610700.032.16493610-11
TAACGCA8100.031.5854284
CGCATAG10750.029.927321
AGTACTT439800.029.66762212-13
GTACATG377750.029.4141921
CATGGGG157800.029.2966084
ACATGGG363950.029.230023
CATGGGT69750.029.0026744
TACATGG384550.028.8131352
Produced by FastQC (version 0.11.5)