FastQCFastQC Report
Fri 10 Feb 2017
SRR3559671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433719
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT495162.034581642334222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239970.9860218044893433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152470.6264897467620543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94310.3875139241629786No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83540.3432606640290025No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.23531886795476387No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.16000203803315008No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.15375645257320175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36670.15067474922125357No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT26530.10901011990291402No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26050.10703782975766717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA213150.054.4459951
TATCACG3650.048.9092182
CGCATAG5600.047.872581
TATCAAC269450.042.931932
TAACGCA4600.042.6848564
ATCAACG274250.042.153633
TCAACGC282200.041.747064
CAACGCA285200.041.4748235
AACGCAG294450.041.262266
ACGCAGA325250.037.3914577
CGCAGAG330400.036.9887128
GCAGAGT345050.035.349289
ACAACGC5400.035.2585953
CATGGGT39700.035.070594
TCACGCA5350.033.3645444
GGTATCA114700.030.540511
GTATCAC8150.029.9701271
CATGGGG70450.029.644484
GTATAAC7450.029.587471
ACATGGG203250.029.1566493