FastQCFastQC Report
Fri 10 Feb 2017
SRR3559670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5565549
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT964521.7330186114613313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466080.8374376004954767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281410.5056284654038622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163680.2940949760751365No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132990.2389521680610484No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98540.17705351260046404No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72050.12945712992554734No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70070.1258995294085094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA334550.067.704421
TATCAAC450050.049.9769672
ATCAACG450350.049.872693
TCAACGC457850.049.5885124
CAACGCA462800.049.1481175
AACGCAG481450.048.0969546
GGTATCA143200.045.0974771
ACGCAGA532100.043.518667
CGCAGAG544550.042.6325728
GCAGAGT581200.039.94429
AGAGTAC556800.031.89072410-11
CGCATAG9550.030.5674131
GTACATG376250.029.8470081
TATCACG7400.029.7515012
AGTACTT375400.029.67697512-13
ACATGGG369750.029.2376673
TACATGG385150.029.0755922
CATGGGT87800.028.9353894
GAGTACT347050.027.48963412-13
CATGGGG158100.027.3588924
Produced by FastQC (version 0.11.5)