FastQCFastQC Report
Fri 10 Feb 2017
SRR3559669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855809
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514451.8014159910554242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247260.8658142053617731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156070.5465001335873653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95350.3338808722852264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90660.3174582053631738No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59520.20841729961632588No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.13558329706223352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37870.1326069075347826No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37330.13071602477616676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA210800.055.4823881
TAACGCA5850.049.8344084
TATCACG4500.043.6381762
TATCAAC273200.042.7131962
ATCAACG273000.042.6934173
TCAACGC278950.042.635914
CAACGCA283350.042.120825
AACGCAG296250.041.4314356
CGCATAG5700.039.720451
TACAACG6150.039.671072
ACGCAGA326950.037.4319157
CGCAGAG333050.036.764198
GCAGAGT345800.035.3398369
GTATAAC9500.035.1212431
ACAACGC7750.033.0107843
GGTATCA114550.032.5600241
CATGGGG87150.028.672864
AGAGTAC339300.028.34529510-11
AGTACTT217750.028.03356212-13
GTACAAC11800.027.7706571