FastQCFastQC Report
Fri 10 Feb 2017
SRR3559668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275456
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189281.4840182648401825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92520.7253876260725576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62240.4879823372974058No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.3551670931807918No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38110.29879509759646744No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26120.20478950273470822No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21000.16464699683877768No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.11580172110994029No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.1141552511415525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2900.049.242194
GTATCAA103300.044.7468571
TATCACG2150.044.2850072
CGCATAG3200.039.0904271
TACAACG2750.038.950682
TCAACGC129850.035.787654
ATCAACG127550.035.778493
CAACGCA130650.035.614065
AACGCAG134800.035.5328566
TATCAAC129550.035.27762
ACAACGC2950.034.2873573
ATCACGC2900.032.8268433
ACGCAGA148600.032.2730757
CGCAGAG151500.031.851688
GCAGAGT158450.030.4170389
GTATAAC5100.029.1991961
TCTAACG1055.901379E-428.3371322
AGATTAC5250.028.3371322
CATGGGT14550.028.2169574
GATTACT5400.027.5456683