FastQCFastQC Report
Fri 10 Feb 2017
SRR3559667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627168
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT694461.9146066573150184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335560.9251294673971538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208740.5754902998703121No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128000.35289239428667213No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97620.26913559008019483No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71200.1962963943219614No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.14636763447405798No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51220.14121209715127614No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48880.13476078306822292No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38830.10705321617305844No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36870.10164955138554377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA285850.055.2920461
TATCAAC368250.042.754662
ATCAACG370400.042.421493
TCAACGC378400.041.98064
CAACGCA382500.041.67065
AACGCAG393700.041.255876
ACGCAGA435350.037.2542467
CGCAGAG443150.036.746218
CGCATAG6700.035.5671271
GCAGAGT464050.035.01439
CATGGGT55650.033.3563044
GGTATCA152650.031.6510141
TATCACG4200.031.168062
ACATGGG287750.029.3603673
TAACGCA6150.029.0224864
CATGGGG109550.028.6211284
AGTACTT284800.028.30660412-13
GTACATG321000.028.0429081
AGAGTAC451200.027.96131910-11
TACATGG321100.027.9445692