FastQCFastQC Report
Fri 10 Feb 2017
SRR3559666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6028082
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1265612.099523530038244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612581.016210462963178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369760.6133957699978202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228700.3793909903680806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186410.3092360057477652No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130150.21590615389770743No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT127640.21174230874762487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99090.1643806437934985No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92500.15344847664646896No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC87630.14536962171383866No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC82250.13644472653158998No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT81080.13450381066481842No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA70670.11723463615790229No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA65810.1091723702497743No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT61550.10210544581178557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA422500.071.570771
TATCAAC569300.053.0062682
ATCAACG576350.052.2145653
TCAACGC586850.051.7771154
CAACGCA597350.051.0363245
GGTATCA170400.050.8068661
AACGCAG614600.050.30096
ACGCAGA688050.044.9831127
CGCAGAG700950.044.2397778
GCAGAGT738800.041.9008149
GACCGTT16050.035.216627
TACTGGT53950.033.5318032
GTACTGG61950.033.1821251
AGAGTAC721650.032.8917810-11
GTACATG468500.030.9681851
ACATGGG454000.030.81033
TACATGG475050.030.5901172
TAACGCA8700.030.090724
CATGGGG217000.029.8036164
GGTTCAC60600.029.1596936
Produced by FastQC (version 0.11.5)