FastQCFastQC Report
Fri 10 Feb 2017
SRR3559665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020265
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608712.015419176794089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291750.9659748399560966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184920.6122641556287279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116430.38549597469096253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97570.3230511229974853No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70270.23266170352601512No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63930.21167016801505828No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.16280028408103261No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45160.14952330341873973No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45010.14902665825680858No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31970.10585163884626017No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30930.10240823239020416No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30930.10240823239020416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6500.056.8339271
GTATCAA285350.049.9891361
TAACGCA5400.045.175264
TATCAAC352100.040.408662
ATCAACG357150.039.7993553
TCAACGC365300.039.5140654
CAACGCA368350.039.4130175
AACGCAG379850.039.1282926
TATCACG5100.038.5038952
ACGCAGA414450.035.8042767
CGCAGAG421350.035.343868
GCAGAGT440200.033.735789
AGATTAC14600.031.3833142
ATAGTAC11750.030.8889054
GTATAAC10300.030.6597061
GCATAGT12250.030.6030922
TCACGCA6750.029.9699294
CATGGGG82350.029.5508694
ACAACGC7250.029.5443943
TAGTACT12450.029.1521825
Produced by FastQC (version 0.11.5)