FastQCFastQC Report
Fri 10 Feb 2017
SRR3559664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410458
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT837732.4563563017049326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406851.1929482785010108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249620.7319251549205414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145170.4256613041415552No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117450.34438189826703625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.2444539706983637No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81790.23982116184981606No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64210.18827383301597617No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63040.18484320874205165No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62590.18352373786746531No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44420.13024643610916775No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC40510.11878170028776194No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39420.11558564861376391No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36250.10629070934167785No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35790.10494191689210072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA368600.051.451431
CGCATAG6050.043.332471
TATCAAC458050.041.3385662
ATCAACG470100.040.2350653
TCAACGC478350.039.9391634
CAACGCA484300.039.4970445
AACGCAG495050.039.4325836
TATCACG6250.038.0844882
ACGCAGA541050.035.9700667
CGCAGAG546050.035.672348
TAACGCA6050.035.403944
TACAACG6200.034.5524562
GCAGAGT565850.034.4871949
AGTACTT351800.029.32525412-13
GGTATCA209550.027.438211
AGAGTAC555050.027.34410310-11
GTATAAC8500.027.3376921
GAGTACT323450.027.05789812-13
ACAACGC8050.026.6079273
TCACGCA9650.025.8956284
Produced by FastQC (version 0.11.5)