FastQCFastQC Report
Fri 10 Feb 2017
SRR3559663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618314
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT808523.0879413240734306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380501.4532252434200024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245040.935869418259231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143370.5475661055167562No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91660.3500726039733966No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71950.274795154439078No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.22483934318038248No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.21567313927970444No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44480.1698803122925669No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC41080.1568948567666063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36010.13753125102642386No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC34450.13157321849098313No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA32690.12485133563048588No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28230.10781747338172579No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT27700.10579327002032606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA269250.062.7924461
TATCAAC346350.048.866212
ATCAACG353850.047.7195363
TCAACGC359750.047.3007744
CAACGCA363400.046.842065
AACGCAG371600.046.384816
ACGCAGA409050.042.1381157
GGTATCA127900.042.1064451
CGCAGAG415500.041.541278
GCAGAGT433900.039.6973889
GACCGTT6800.033.2489367
TATCACG3600.033.061442
ACGGTAC6850.033.006243
GGTTCAC22050.032.3799136
GTACTGG26350.030.7474441
AGAGTAC426300.030.67019710-11
TACTGGT24300.030.3675442
GGACCGT10900.030.021976
AGTACTT308300.029.5367612-13
CATGGGG85500.029.4358984
Produced by FastQC (version 0.11.5)