FastQCFastQC Report
Fri 10 Feb 2017
SRR3559661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3637681
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT663671.8244315540587535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304650.8374841004475103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198500.5456773147507986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120060.3300454327908357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83960.23080638461701286No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64590.17755817511211125No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48160.13239203767455146No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47850.13153984640214467No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37730.10371992486421981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA251800.061.449931
TATCAAC325700.047.4281852
ATCAACG328950.046.7548333
TCAACGC333200.046.5513084
CAACGCA337800.045.987855
AACGCAG348300.045.3189286
ACGCAGA386550.040.834527
CGCAGAG397850.039.7788438
GCAGAGT420850.037.5624669
GGTATCA120100.036.8596341
TAACGCA5700.030.2704584
AGAGTAC406500.029.61641710-11
GTACATG278950.029.3044031
ACATGGG263850.029.2242763
CATGGGG115550.028.7316194
AGTACTT267650.028.72004712-13
CGCATAG6050.028.5593171
TACATGG284350.028.5228022
GAGTACT245750.027.13950512-13
TACAACG7100.026.8189142
Produced by FastQC (version 0.11.5)