FastQCFastQC Report
Fri 10 Feb 2017
SRR3559660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428189
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT815601.5025269016977854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394890.7274801964338382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247220.45543734752050824No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141780.26119208450553216No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105860.19501900173335895No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78110.14389697926877637No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59150.10896820283892104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA325050.060.586021
TATCAAC423200.046.4469832
ATCAACG422150.046.3980373
TCAACGC426750.046.3584
CAACGCA432100.045.92135
AACGCAG446050.045.1649976
ACGCAGA492850.040.8762257
CGCAGAG504600.039.9715548
GCAGAGT536850.037.470619
GGTATCA157250.034.6722151
TAACGCA7050.031.2262024
AGAGTAC516900.029.54730810-11
AGTACTT329100.028.27544612-13
GTACATG376850.027.9711571
ACATGGG359200.027.8444063
TACATGG382400.027.4676442
GAGTACT297200.026.61583512-13
CATGGGT56650.025.836984
CATGGGG154900.025.0055334
TACTTTT376350.024.74934814-15
Produced by FastQC (version 0.11.5)