FastQCFastQC Report
Fri 10 Feb 2017
SRR3559659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565770
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT831162.3309411431472022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396431.1117654812284585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247970.6954178199939984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151700.42543405772105325No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119930.3363368921719572No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83810.23504039800660168No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72260.20264907719791236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64540.18099877445825166No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61870.1735109106868923No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53940.15127167484161907No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51690.1449616772814848No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43630.12235786379940378No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39280.11015853518314418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7300.054.681581
GTATCAA353250.053.5320471
TAACGCA6800.046.3729744
TATCAAC441800.042.6543162
ATCAACG450050.041.8682983
TCAACGC460050.041.397934
CAACGCA465750.041.0190355
AACGCAG478300.040.626916
ACGCAGA525550.037.042247
CGCAGAG532050.036.6344268
GTATAAC10050.036.1620371
TACAACG7650.035.7797242
GCAGAGT553550.035.147059
GTACTGG32300.031.9104331
ATAGTAC12900.031.3629684
ATAACGC11850.031.129443
GACCGTT7550.030.7337577
TACTGGT27900.030.4980752
ACAACGC9200.030.395413
GGTATCA195300.030.2315541